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htt plasmids p426 25q gpd  (Addgene inc)


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    Addgene inc htt plasmids p426 25q gpd
    Htt Plasmids P426 25q Gpd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p426+25q+gpd/p426+25Q+GPD+(Plasmid+%231181)/pm37732342-56-25-42
    Average 93 stars, based on 7 article reviews
    htt plasmids p426 25q gpd - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Integrated multi-omics analysis of Huntington disease identifies pathways that modulate protein aggregation
    Article Snippet: Collected cell pellets were resuspended in 25 ml of electroporation buffer [0.1 M lithium acetate (#GRM1507), 10 mM Dithiothreitol (DTT) (#MB070) 10 mM Tris-HCl (#MB030), 1 mM EDTA (#RM1370, ddH2O) (all by Himedia), and left to incubate in a shaking incubator at 30°C, 200 rpm for 1 h. Cells were then spun down (for 5 min at 1500 g ), washed and resuspended in 200 μl 1 M sorbitol. .. A total of 8×10 8 cells was taken for transformation with p426 25Q GPD, p426 46Q GPD, p426 72Q GPD and p426 103Q GPD (Addgene plasmids #1181 , #1182 , #1183 and #1184 , respectively). .. 8×10 8 cells were incubated on ice with 2 μg/ml of plasmid DNA for 5 min and transferred into a 0.2 cm electroporation cuvette (Bio-Rad, #1652086).

    Article Title: Vitamin B 6, B 12 and folate modulate deregulated pathways and protein aggregation in yeast model of Huntington disease.
    Article Snippet: .. Yeast culture, transformation and supplementation study Yeast strain BY4741 and BY4742 were revived and transformed with p426 25Q GPD and p426 72Q GPD plasmid (Purchased from addgene, Addgene plasmid # 1181, 1183; http:// n2t. net/ addge ne: 1181/ 1183; RRID: Addgene_1181/1183) (Krobitsch and Lindquist 2000). ..

    other:

    Article Title: Protein quality control during aging involves recruitment of the macroautophagy pathway by BAG3
    Article Snippet: Following vector linearisation with BamH1 and Not1 (for pBAG3-N1) or BamH1 only (for pBAG3.EGFP-N1) PCR products were inserted using the In-Fusion reaction according to manufacturer's protocol (Clontech).

    Plasmid Preparation:

    Article Title: Vitamin B 6, B 12 and folate modulate deregulated pathways and protein aggregation in yeast model of Huntington disease.
    Article Snippet: .. Yeast culture, transformation and supplementation study Yeast strain BY4741 and BY4742 were revived and transformed with p426 25Q GPD and p426 72Q GPD plasmid (Purchased from addgene, Addgene plasmid # 1181, 1183; http:// n2t. net/ addge ne: 1181/ 1183; RRID: Addgene_1181/1183) (Krobitsch and Lindquist 2000). ..



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    htt plasmids p426 25q gpd  (Addgene inc)
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    Addgene inc htt plasmids p426 25q gpd
    Htt Plasmids P426 25q Gpd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p426+25q+gpd/p426+25Q+GPD+(Plasmid+%231181)/pm37732342-56-25-42
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    p426 72q gpd plasmid  (Addgene inc)
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    Addgene inc p426 72q gpd plasmid
    P426 72q Gpd Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p426 25q gpd  (Addgene inc)
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    p426 46q gpd  (Addgene inc)
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    Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models <t>46Q,</t> 72Q and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.
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    p426 72q gpd  (Addgene inc)
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    Addgene inc p426 72q gpd
    Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, <t>72Q</t> and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.
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    constitutive gpd p426 gpd p  (Addgene inc)
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    Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, <t>72Q</t> and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.
    Constitutive Gpd P426 Gpd P, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p426  (Addgene inc)
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    Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, <t>72Q</t> and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.
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    25q gpd  (Addgene inc)
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    Addgene inc 25q gpd
    Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, <t>72Q</t> and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.
    25q Gpd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, 72Q and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.

    Journal: Disease Models & Mechanisms

    Article Title: Integrated multi-omics analysis of Huntington disease identifies pathways that modulate protein aggregation

    doi: 10.1242/dmm.049492

    Figure Lengend Snippet: Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, 72Q and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.

    Article Snippet: A total of 8×10 8 cells was taken for transformation with p426 25Q GPD, p426 46Q GPD, p426 72Q GPD and p426 103Q GPD (Addgene plasmids #1181 , #1182 , #1183 and #1184 , respectively).

    Techniques:

    Metabolite set enrichment analysis (MESA) and shared deregulated pathways. (A) Yeast metabolomics (present study) show significantly different levels of metabolites in yeasts 46Q, 72Q and 103Q compared to 25Q. (B) MSEA showing shared pathways deregulated in 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q. (C) Metabolomics pathway overlaps determined in our study (yeasts 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q) and literature show pathways commonly deregulated in yeast 103Q compared to yeast 25Q. (D,E) Comparative analysis of significantly enriched metabolic pathways identified in a HD mouse model (D) and in HD patients (E). (F) Metabolomics of previously published deregulated metabolic pathways in human (literature) and deregulated pathways identified in our HD patient cohort (present study), showing an overlap of three deregulated pathways. (G) Plotted is the overlap of deregulated metabolic pathways identified in yeast and mouse HD models, and HD patients. BS, brain stem; CB, cerebellum; CSF, cerebrospinal fluid; CT, cortex; FL, frontal lobe; ST, striatum.

    Journal: Disease Models & Mechanisms

    Article Title: Integrated multi-omics analysis of Huntington disease identifies pathways that modulate protein aggregation

    doi: 10.1242/dmm.049492

    Figure Lengend Snippet: Metabolite set enrichment analysis (MESA) and shared deregulated pathways. (A) Yeast metabolomics (present study) show significantly different levels of metabolites in yeasts 46Q, 72Q and 103Q compared to 25Q. (B) MSEA showing shared pathways deregulated in 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q. (C) Metabolomics pathway overlaps determined in our study (yeasts 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q) and literature show pathways commonly deregulated in yeast 103Q compared to yeast 25Q. (D,E) Comparative analysis of significantly enriched metabolic pathways identified in a HD mouse model (D) and in HD patients (E). (F) Metabolomics of previously published deregulated metabolic pathways in human (literature) and deregulated pathways identified in our HD patient cohort (present study), showing an overlap of three deregulated pathways. (G) Plotted is the overlap of deregulated metabolic pathways identified in yeast and mouse HD models, and HD patients. BS, brain stem; CB, cerebellum; CSF, cerebrospinal fluid; CT, cortex; FL, frontal lobe; ST, striatum.

    Article Snippet: A total of 8×10 8 cells was taken for transformation with p426 25Q GPD, p426 46Q GPD, p426 72Q GPD and p426 103Q GPD (Addgene plasmids #1181 , #1182 , #1183 and #1184 , respectively).

    Techniques:

    Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, 72Q and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.

    Journal: Disease Models & Mechanisms

    Article Title: Integrated multi-omics analysis of Huntington disease identifies pathways that modulate protein aggregation

    doi: 10.1242/dmm.049492

    Figure Lengend Snippet: Metabolomic study using an HD yeast model. (A-F) Heat maps of targeted metabolomics in Htt yeast models 46Q, 72Q and 103Q compared to that of 25Q in positive and negative modes. Altered metabolites in 46Q compared to 25Q in positive (A) and negative (B) mode. Altered metabolites in 72Q compared to 25Q in positive (C) and negative mode (D). (E) Altered metabolites in 103Q compared to 25Q in positive (E) and negative (F) mode.

    Article Snippet: A total of 8×10 8 cells was taken for transformation with p426 25Q GPD, p426 46Q GPD, p426 72Q GPD and p426 103Q GPD (Addgene plasmids #1181 , #1182 , #1183 and #1184 , respectively).

    Techniques:

    Metabolite set enrichment analysis (MESA) and shared deregulated pathways. (A) Yeast metabolomics (present study) show significantly different levels of metabolites in yeasts 46Q, 72Q and 103Q compared to 25Q. (B) MSEA showing shared pathways deregulated in 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q. (C) Metabolomics pathway overlaps determined in our study (yeasts 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q) and literature show pathways commonly deregulated in yeast 103Q compared to yeast 25Q. (D,E) Comparative analysis of significantly enriched metabolic pathways identified in a HD mouse model (D) and in HD patients (E). (F) Metabolomics of previously published deregulated metabolic pathways in human (literature) and deregulated pathways identified in our HD patient cohort (present study), showing an overlap of three deregulated pathways. (G) Plotted is the overlap of deregulated metabolic pathways identified in yeast and mouse HD models, and HD patients. BS, brain stem; CB, cerebellum; CSF, cerebrospinal fluid; CT, cortex; FL, frontal lobe; ST, striatum.

    Journal: Disease Models & Mechanisms

    Article Title: Integrated multi-omics analysis of Huntington disease identifies pathways that modulate protein aggregation

    doi: 10.1242/dmm.049492

    Figure Lengend Snippet: Metabolite set enrichment analysis (MESA) and shared deregulated pathways. (A) Yeast metabolomics (present study) show significantly different levels of metabolites in yeasts 46Q, 72Q and 103Q compared to 25Q. (B) MSEA showing shared pathways deregulated in 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q. (C) Metabolomics pathway overlaps determined in our study (yeasts 25Q vs 46Q, 25Q vs 72Q and 25Q vs 103Q) and literature show pathways commonly deregulated in yeast 103Q compared to yeast 25Q. (D,E) Comparative analysis of significantly enriched metabolic pathways identified in a HD mouse model (D) and in HD patients (E). (F) Metabolomics of previously published deregulated metabolic pathways in human (literature) and deregulated pathways identified in our HD patient cohort (present study), showing an overlap of three deregulated pathways. (G) Plotted is the overlap of deregulated metabolic pathways identified in yeast and mouse HD models, and HD patients. BS, brain stem; CB, cerebellum; CSF, cerebrospinal fluid; CT, cortex; FL, frontal lobe; ST, striatum.

    Article Snippet: A total of 8×10 8 cells was taken for transformation with p426 25Q GPD, p426 46Q GPD, p426 72Q GPD and p426 103Q GPD (Addgene plasmids #1181 , #1182 , #1183 and #1184 , respectively).

    Techniques: